Note: a new version of plusTipTracker will be released in 2013 with a new interface. The download bundle includes a user's guide in the form of a Technical Report and complete documentation of all the functions in the package. We strongly recommend the download of this package for the purpose of microtubule tracking. It contains a sizable set of service functions for data visualization and statistical processing of parameters of microtubule dynamics. The package can be operated via a convenient graphical user interface. Concepts are related to the ClusterTrack software however, the tracking builds on the algorithms implemented in the u-track software. PlusTipTracker is a MATLAB software package for tracking the full dynamics of microtubules based on +TIP marker live cell image sequences. Some features require third-party software (Imaris).įor more detail, see the Users Guide or Elliott et al., Nat Cell Bio 17, 137-147 (2015) It does not have a point-and-click graphical user interface and requires some familiarity with MATLAB, as well as specific data formats. This software allows the analysis of cell shape (both branching and local surface curvature) and motion, and correlation between these shape and motion parameters and subcellular fluorescence localization. It identifies and computationally tracks speckles and utilizes their location, appearance, and disappearance to derive network flows and assembly/disassembly maps.įor more information, please see Mendoza et al., Current Protocols in Cytometry 62:2.18.1–2.18.26 (2012). QFSM (Quantitative Fluorescent Speckle Microscopy) is a MATLAB software for quantifying the rate of polymer assembly, disassembly and flow across the entire leading edge in constitutively motile cells from fluorescent speckle microscopy movies. Quantitative Fluorescent Speckle Microscopy* Decoupling global biases and local interactions between cell biological variables
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